My Notes
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This paper investigates factors that influence substrate specificity (O2 versus NO reductase activity) within flavodiiron enzymes. The authors mutate potentially relevant amino acids and investigate the biochemical and biophysical properties by UV-visible and EPR spectroscopies.
| Attachment | Size |
|---|---|
| Flavodiiron Oxygen Reductase_0.docx | 43.24 KB |
| Flavodiiron Oxygen Reductase_0.pdf | 153.86 KB |
Upon completion of this assignment, a student should be able to:
- Read, interpret, and summarize data and results from a primary literature article
- Determine whether samples will be EPR active based on electron configurations and coupling
- Explain the purpose and outcomes of specific analytical techniques in studies of a metalloprotein
- Understand the role of individual amino acids in directing substrate specificity in a metalloprotein
This article was used as part of a literature exam for a Jr/Sr special topics course in bioinorganic chemistry. Students were given the paper to read before the exam to prepare, and then given a clean copy of the article to use during the in-class exam.
Evaluation
Students were graded based on the provided answer key.
I no longer have student-specific data, but my recollection is that students did well overall on this assessment.
Comments
Excellent assignment, Amanda.