In this experiment, students will synthesize and characterize one of three Ag(I) cyanoximate complexes as potential antimicrobial agents for use in dental implants. This experiment combines simple ligand synthesis, metalation and characterization, and a biomedical application. The complexes are both air and light stable. Students apply the Kirby-Bauer disk diffusion test, a common microbiology assay, to determine the antibacterial properties of their complexes. Students will also perform a simple cost analysis as part of the evaluation of the complexes. This experiment was designed during the June 2015 “Improving Inorganic Chemistry Pedagogy” workshop funded by the Associated Colleges of the South.
A student should be able to:
- Prepare one of a series of Ag(I) cyanoximate complexes and perform appropriate characterization of identity and purity
- Measure antimicrobial activity in a semi-quantitative way using the Kirby-Bauer assay, including design and implementation of appropriate control experiments.
- Evaluate a series of complexes as potential antimicrobials for dental applications based on the criteria of heat stability, water insolubility, and antibacterial activity.
- Identify most cost effective complex.
Melting point apparatus
In this experiment, students connect organic synthesis, inorganic synthesis, and applications in microbiology in a multiweek experiment.
Students synthesize one of three possible derivatives of a cyanoxime ligand, coordinate Ag(I), and test the antimicrobial properties of their compound. The antimicrobial assay requires supplies not commonly found in a chemistry laboratory, and instructors are encouraged to collaborate with a colleage in microbiology.
This experiment has been tested several times since it was first developed in 2015. Additional notes were added in January 2020. We welcome others in the VIPEr community to help us test this experiment. If you do try it, please consider posting your comments or filling out our evalution survey: